Product Description The Microarray Slide Imprinter is an easy and cost effective way to partition microarray slides and substrates with a hydrophobic pattern for multiplexed microarray applications. This durable and fun to use instrument is perfect for setting up high throughput microarray experiments where multiple assays need to be run on a single microarray slide/substrate with minimal risk of contamination between different assays. imprint hydrophobic patterns onto any slide and surface (any design/pattern can be imprinted) empowers high throughput sample processing pays for itself quickly by amortizing surface chemistry cost over many microarray experiments on a single slide/substrate patterns meet micro spotting pin spacing of all Stealth and 946 printheads and microarrayer programming parallel loading of samples using multi-channel pipettes or automated pipetting liquid systems such as Oasis and others does not contribute to background or light scattering in scanning for colorimetric, CCD and laser/pmt fluorescent microarray scanners stays on throughout all microarray processing and detection steps compatible with a wide variety of aqueous and solvent based buffers works for both DNA, protein, carbohydrate, small molecule and other types of microarrays easy to use, no setup or assembly required fast, each slide only takes a few seconds to imprint fun to use works with any and all microarray surface chemistry including hydrogels and membrane based surfaces such as SuperNitro and SuperProtein inexpensive, low cost to operate and own

How the Microarray Slide Imprinter works

The Microarray Slide Imprinter provides a fast and convenient way to define multiple “wells” on a single microscope slide size substrate to create separated regions to process many assays on a single substrate. This is accomplished by imprinting a thin, inert, hydrophobic film patterned onto the surface of a slide/substrate through the use of a pre-designed “stamp.”

Step 1. Select stamp and make sure it is immersed in a bath of hydrophobic film polymer.

Step 2. A microscope slide size substrate is inserted into a cartridge above the bath and held accurately for consistent positioning. Microarray locations are consistent from slide to slide.

Step 3. A lever on the outside of the imprinter is pulled so that the “stamp” can touch off on the surface of the substrate, thus imprinting the desired hydrophobic barrier lines onto the substrate. Microarrays can be now be manufactured and processed.

Since the printed hydrophobic pattern printed is thin, the imprinted slides/substrates can be inserted directly into microarrayers and microarray processing racks and wash stations and directly into a microarray scanners without any further modifications. The coating is highly hydrophobic, does not dissolve in most solvent systems, and will stay on the slide through multiple wash steps and overnight incubations. The Microarray Slide Imprinter offers excellent flexibility. The imprinted hydrophobic barriers can be made in any design and works with all microarray surface chemistries including membranes and hydrogels. Additionally the barrier shows excellent stability in most solvents through repeated washes, is chemically inert and does not contribute to light scatter or background noise in detection.

Image of a 12 well imprinted slide using food coloring for visual effect to show how the different areas of the slide will not cross contaminate.