NanoPrint™ Microarrayer
	NanoPrint™ Protein Edition
	Microplate Microarrayer

NanoPrint™ Microarrayer

MICROARRAYING SYSTEM

Automation Transcribed by Function...

The NanoPrint™ Microarray System is a robust and customizable platform for all microarray applications in Genomics, Proteomics and Diagnostics. The NanoPrint™ systems manufactures high quality, precision microarrays utilizing the ArrayIt brand patented Stealth Micro Spotting Device, superior linear drive motion control technology, and proprietary Warp1 controllers from Dynamic Devices.

Compatible with all standard microarray surfaces (25 x 76 x 1mm), the system can easily be configured to print microarrays into the flat bottoms of 96 and 384 well plates by taking advantage of it’s flexible deck configuration and easy to use software interface. The Stealth Micro Spotting Device is capable of printing DNA, RNA, cDNA, BACS, Oligonucleotides, Antibodies, Antigens, Proteins, Lipids, Carbohydrates, Receptors, Small Cells and many other biomolecules that can be put into suspension onto a solid surface.

The ArrayManager Software combines unparalleled power and simplicity into a graphical Windows* based package. To view the ArrayManager Software screen shot tutorial, please clic on this link to Arrayit's website

Features include:

• A Method Creation Wizard,
• User & version control management,
• Auto Calibration of the slide and micro plate positions
• Complete sample tracking
• Support of input / output data files
• Custom array designs
• Speed profiles and wash protocols
• Automatic Method Validation
• Runtime sample and spotting views as well as a Simulation Mode
• Easy to use graphical Re-Print Wizard

The high speed, high precision linear servo control system of the NanoPrint™, results into superior instrument performance in both speed and precision. Combined with the efficient bench top design, user configurable worktable, humidity & dust control, host of available options and the flexible & sophisticated ArrayManager Software, the NanoPrint™ system is the complete solution for high performance microarray printing.

The value of the linear servo motor technology used on the NanoPrint™ Microarrayer:

• Nanometer scale positional accuracy
• Quiet operation
• No vibration
• No heat generation
• No motor dust
• Superior Accuracy
• Maintenance Free
• High Speed
• High Load capacity
• Zero "sagging"
• Safer than stepper motor drives

Specifications:

Samples of NanoPrint™ deck configurations :

Example of NanoPrint™ configuration (210 slides, 50 plate stacker) :

How to Purchase this system:

• Flexible deck configurations
• Numbers of slides
• Wash station options
• And special environmental controls specific to protein microarraying

NanoPrint™ Protein Edition

The NanoPrint™ Protein Edition High Throughput Microarrayer includes everything needed to manufacture protein microarrays.

Deliverables specific to the Protein Edition NanoPrint include:

• NanoPrint Microarrayer
• Stealth 48 Micro Spotting Device(with extended collars)
• Multi-station wash, dry station and sonicator for Micro Spotting Pins to remove even the “stickiest” of proteins
• Digitally controlled Plate and/or Slide cooling options
• Digitally controlled humidity chamber
• Hepa Filtered Microarraying Chamber
• ArrayManager software
• A starter pack of consumables to get your started printing your samples immediately including: Microarray Microplates, SuperEpoxy Substrates, SuperProtein Substrates, Protein Printing Buffer, and BlockIt.

* Good reasons to use the "cold room" capabilities of the Protein Edition NanoPrint™ Microarrayer.

Proteins have a number of levels of organization. This includes the primary structure, (amino acid sequence, held together by covalent bonding), the secondary structure (structures such as the beta-pleated sheets and that are held together primarily by hydrogen bonding), the tertiary structure (held together by hydrogen bonding and disulfide bonds) and the quaternary structure (which combines multiple amino acid sequences together by hydrogen, disulfide and other bonding).

Each one of these levels of organization is required for an active protein and required to print high quality protein arrays that can detect protein interactions. In addition, intact epitope structure is a requirement for antibody binding.

Hydrogen bonding, the force that maintains the correct folding of proteins, has temperature dependant labiality. This temperature dependant change in structure results in protein denaturation and inactivation and destruction of proteins. This is most evident when a protein source, such as an egg is heated to high temperatures during cooking. This same effect happens at lower temperatures. For every 10 degrees Celsius the rate of denaturation doubles.

This helps explain why food lasts longer in the refrigerator and why proteins last longer in the cold SpotBot arraying chamber. The optimal temperature for proteins (excluding freezing) is 4C. For the last 30 years protein chemistry has been performed in the cold room - the Protein Edition SpotBot frees you from the cold but saves your proteins.

Another key factor that affects protein integrity is microbial contamination (bacteria and fungi). The metabolic rate of microbes is greatly reduced under cold conditions. This reduces their doubling time and reduces infestation in samples and degradation of proteins.

Microplate Microarrayer : High throughput microarraying into the flat bottoms of 96 well plates.

Cassette Plate Handling Module :

The Microplate Microarrayer system takes advantage of a NanoPrint Microarray Robot coupled with a high speed, bi-directional, modular transportation system designed for the movement of 20 tray cassettes each holding 9 flat bottom 96 well plates per cassette on a storage cart. Users of this system will be empowered to print over 17,000 microarrays, with ~1,000 features with complete automation.

The Microplate Microarrayer is a unique product solution for high throughput printing of microarrays into the bottoms of 96 well plates. The system takes advantage of a modular approach built on reliable and heavy-duty industrial components and electronics. It unique design provides unparalleled speed and proven reliability by eliminating the typical gripper system of microplate handling and issues with tolerance build up that can generate system errors.

Removable storage carts hold the tray cassettes to be processed and can be used interchangeably between multiple systems. The system requires a standard footprint for the tray cassette but allows unlimited flexibility for the variety and combination of custom cassettes that can be created within this footprint. A standard tray cassette can hold up to total of 9 SBS standard plates: microplates, deep well plates, or disposables, however, custom layouts required for specific applications can also be delivered.

Plate Handling Features :
· Unparalleled Speed and Reliability
· Conveyor Based Technology
· Supports Third Party Liquid Handlers as well as theNanoPrint Microarrayer
· Any Combination Samples/Disposables
· Modular and Scalable
· Precise Position Repeatability
· No Modification Required to Instruments

The sample cassette may be located anywhere along the conveyor with precise repeatability by means of out patented cassette stopping system that provides precise sensing and locating capability. A cassette position may be adjusted simply by sliding the cassette stop along the conveyor rail.

For Microarraying Specifications Please see: NanoPrint Microarrayer

Plate Handling Specifications